EEWOWW is an online personal knowledge management tool. Click to find out more!
 
zz
Updated in 2017/2/27 下午 10:02:28      Viewed: 318 times      (Journal Article)
The Journal of biological chemistry 276 (52): 49125-32 (2001)

A response calculus for immobilized T cell receptor ligands.

P S Andersen , C Menné , R A Mariuzza , C Geisler , K Karjalainen
ABSTRACT
To address the molecular mechanism of T cell receptor (TCR) signaling, we have formulated a model for T cell activation, termed the 2D-affinity model, in which the density of TCR on the T cell surface, the density of ligand on the presenting surface, and their corresponding two-dimensional affinity determine the level of T cell activation. When fitted to T cell responses against purified ligands immobilized on plastic surfaces, the 2D-affinity model adequately simulated changes in cellular activation as a result of varying ligand affinity and ligand density. These observations further demonstrated the importance of receptor cross-linking density in determining TCR signaling. Moreover, it was found that the functional two-dimensional affinity of TCR ligands was affected by the chemical composition of the ligand-presenting surface. This makes it possible that cell-bound TCR ligands, despite their low affinity in solution, are of optimal two-dimensional affinity thereby allowing effective TCR binding under physiological conditions, i.e. at low ligand densities in cellular interfaces.
DOI: 10.1074/jbc.M109396200      ISSN: 0021-9258