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Updated in 2017/1/17 下午 05:38:58      Viewed: 594 times      (Journal Article)
Nature medicine 15 (5): 545-52 (2009)

Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism.

Agnes Machnik , Wolfgang Neuhofer , Jonathan Jantsch , Anke Dahlmann , Tuomas Tammela , Katharina Machura , Joon-Keun Park , Franz-Xaver Beck , Dominik N Müller , Wolfgang Derer , Jennifer Goss , Agata Ziomber , Peter Dietsch , Hubertus Wagner , Nico van Rooijen , Armin Kurtz , Karl F Hilgers , Kari Alitalo , Kai-Uwe Eckardt , Friedrich C Luft , Dontscho Kerjaschki , Jens Titze
ABSTRACT
In salt-sensitive hypertension, the accumulation of Na(+) in tissue has been presumed to be accompanied by a commensurate retention of water to maintain the isotonicity of body fluids. We show here that a high-salt diet (HSD) in rats leads to interstitial hypertonic Na(+) accumulation in skin, resulting in increased density and hyperplasia of the lymphcapillary network. The mechanisms underlying these effects on lymphatics involve activation of tonicity-responsive enhancer binding protein (TonEBP) in mononuclear phagocyte system (MPS) cells infiltrating the interstitium of the skin. TonEBP binds the promoter of the gene encoding vascular endothelial growth factor-C (VEGF-C, encoded by Vegfc) and causes VEGF-C secretion by macrophages. MPS cell depletion or VEGF-C trapping by soluble VEGF receptor-3 blocks VEGF-C signaling, augments interstitial hypertonic volume retention, decreases endothelial nitric oxide synthase expression and elevates blood pressure in response to HSD. Our data show that TonEBP-VEGF-C signaling in MPS cells is a major determinant of extracellular volume and blood pressure homeostasis and identify VEGFC as an osmosensitive, hypertonicity-driven gene intimately involved in salt-induced hypertension.
DOI: 10.1038/nm.1960      ISSN: 1078-8956